Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP ® Rabbit mAb (Alexa Fluor ® 647 Conjugate) 4851S from Cell Signaling Technology

Product Specs
ItemPhospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP ® Rabbit mAb (Alexa Fluor ® 647 Conjugate)
CompanyCell Signaling Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog Number4851S
Quantity100 µl
ApplicationsIF-IC, FC-FP
ReactivityH, M, R, Mk, Mi, Sc
TargetS6 Ribosomal Protein (Ser235/Ser236) phosphate
ModificationsS6 Ribosomal Protein (Ser235/Ser236) phosphate
HostRabbit
NCBI Gene AliasesS6, eS6
Conjugate/TagAlexa Fluor ® 647
ClonalityMonoclonal
NCBI Full Gene Nameribosomal protein S6
Research AreaTranslational Control
IsotypeIgG
CloneD57.2.2E

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) A structural blueprint for interleukin-21 signal modulationCell ReportsJune 19, 2023Gita C. Abhiraman, Theodora U.J. Bruun, Nathanael A. Caveney, Leon L. Su, Robert A. Saxton, Qian Yin, Shaogeng Tang, Mark M. Davis, Kevin M. Jude, K. Christopher GarciaAnti-phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate),Cell Signaling Technologies,Cat#4851S; RRID:AB_10695457 Ultra-LEAF™ Purified anti-human CD40 Antibody,BioLegend,Cat#668103; RRID:AB_2814510 Goat Anti-Human Lambda-BIOT

(2) BTG1 mutation yields supercompetitive B cells primed for malignant transformationScienceJanuary 20, 2023Coraline Mlynarczyk, Matt Teater, Juhee Pae, Christopher R. Chin, Ling Wang, Theinmozhi Arulraj, Darko Barisic, Antonin Papin, Kenneth B. Hoehn, Ekaterina Kots, et al.dilution 1:400), AF647 anti-pS6 (S6 phosphorylated at S235/236, clone D57.2.2E, Cell Signaling Technology 4851, dilution 1:200), APC streptavidin (BioLegend 405207, dilution 1:300) and PE-Cy7 streptavidin (eBio-science

(3) Diminished cell proliferation promotes natural killer cell adaptive-like phenotype by limiting FcεRIγ expressionJournal of Experimental MedicineNovember 7, 2022Avishai Shemesh, Yapeng Su, Daniel R. Calabrese, Daniel Chen, Janice Arakawa-Hoyt, Kole T. Roybal, James R. Heath, John R. Greenland, Lewis L. LanierAF647-conjugated anti–Ki-67 (#558615, RRID:AB_647130). Cell Signaling: AF647-conjugated anti–pS6-S235/236 (#4851, RRID:AB_10695457), AF647-conjugated anti–pAKT-T308 (#48646, RRID:AB_2799341), AF647-conjugated anti–pAKT-S473

(4) mTOR-regulated mitochondrial metabolism limits mycobacterium-induced cytotoxicityCellSeptember 29, 2022Antonio J. PagÃ¡n, Lauren J. Lee, Joy Edwards-Hicks, Cecilia B. Moens, David M. Tobin, Elisabeth M. Busch-Nentwich, Erika L. Pearce, Lalita RamakrishnanProtein (S235/S236) XP rabbit (clone D57.2.2E) (Alexa Fluor 647 Conjugate),Cell Signaling Technology,Cat# 4851;RRID:AB_10695457 S6 Ribosomal Protein (clone 54D2) Mouse mAb (Alexa Fluor 647 Conjugate),Cell Signaling Technology

(5) Differential IL-12 signaling induces human natural killer cell activating receptor-mediated ligand-specific expansionJournal of Experimental MedicineAugust 1, 2022Avishai Shemesh, Harry Pickering, Kole T. Roybal, Lewis L. LanierAF-647–conjugated anti–Ki-67 (cat. 558615). Cell Signaling: AF-647–conjugated anti-pS6S235/236 (cat. 4851S), PE-conjugated pBadS112 (cat. 11865S), Biotin-conjugated Bcl-xL (cat. 87979S), AF594-conjugated Mcl-1

(6) Carbon Monoxide Activates PERK-Regulated Autophagy to Induce Immunometabolic Reprogramming and Boost Antitumor T-cell FunctionCancer researchMay 16, 2022Chakraborty P, Parikh RY, Choi S, Tran D, Gooz M, Hedley ZT, Kim DS, Pytel D, Kang I, Nadig SN, et al.3179: Phospho-S6 Ribosomal Protein (Ser235/236)- Alexa647,Cell Signaling Technology,Clone: D57.2.2E; Cat# 4851; RRID: AB_916160 IRE1 α,Cell Signaling Technology,Clone: 14C10; Cat#3294 Anti-Rabbit HRP,Cell Signaling Technology

(7) Germinal center expansion but not plasmablast differentiation is proportional to peptide-MHCII density via CD40-CD40L signaling strengthCell reportsMay 3, 2022Jing Z, McCarron MJ, Dustin ML, Fooksman DR.17-9852-82; RRID:AB_2573318 Rabbit monoclonal anti-PhosphoS6 (Ser235/236) AF647 (clone D57.2.2E),CST,Cat# 4851S; RRID:AB_10695457 Rat monoclonal anti-TCR Vα2 AF647 (clone B20.1),BioLegend,Cat# 127812; RRID:AB_1186115 DEC-OVA,This

(8) Intracellular metabolic adaptation of intraepithelial CD4+CD8Î±Î±+ T lymphocytesiScienceApril 15, 2022Yosuke Harada, Tomohisa Sujino, Kentaro Miyamoto, Ena Nomura, Yusuke Yoshimatsu, Shun Tanemoto, Satoko Umeda, Keiko Ono, Yohei Mikami, Nobuhiro Nakamoto, et al.AB_11147170 Phospho-S6 ribosomal protein (Ser235/236) (clone: D57.2.2E; Alexa647),Cell Signaling,Cat# 4851; RRID: AB_10695457 ,, "Chemicals, peptides, and recombinant proteins","Chemicals, peptides, and recombinant

(9) CDK8 Fine-Tunes IL-6 Transcriptional Activities by Limiting STAT3 Resident Time at the Gene LociSSRN Electronic JournalMarch 31, 2022Jonathan Martinez-Fabregas, Luopin Wang, Elizabeth Pohler, adeline cozzani, Stephan Wilmes, Majid Kazemian, Ignacio MoragaCell Signaling Cat#5018S; RRID: AB_10695861 Rabbit anti-pS6R-S235/S236-AF647 (Clone D57.2.2E) Cell Signaling Cat#4851S; RRID: AB_10695457 Rabbit anti-pZAP70-Y319/pSYK-Y352AF647 (Clone 65E4) Cell Signaling Cat#82975S;

(10) CD137 (4-1BB) costimulation of CD8+ T cells is more potent when provided in cis than in trans with respect to CD3-TCR stimulationNature CommunicationsDecember 15, 2021Itziar Otano et al.Cells were then permeabilized with cold Perm III Buffer (558050, BD). Then, anti-phospho-S6-AF647 (4851, Cell Signaling) was detected by intranuclear staining. Mouse T cells were surface stained with the

(11) Phenotypic characterization of patients with activated PI3KÎ´ syndrome 1 presenting with features of systemic lupus erythematosusGenes & DiseasesNovember 1, 2021Yanping Wang, Qiuyun Yang, Xuemei Chen, Wenjing Tang, Lina Zhou, Zhi Chen, Yunfei An, Zhiyong Zhang, Xuemei Tang, Xiaodong ZhaoPerm buffer III (both from BD Biosciences) and stained with the following antibodies: anti-phospho-S6 (#4851, Cell Signaling Technology, Danvers, MA, USA) and anti-CD3 (clone SK7, BioLegend). Western blot T cells

(12) Detection of phenotype‐specific therapeutic vulnerabilities in breast cells using a CRISPR loss‐of‐function screenMolecular OncologyAugust 1, 2021Anna Barkovskaya et al.samples were washed and pooled for staining with anti‐phospho‐S6 Alexa 647 (Cell Signaling Technology, #4851 dilution 1 : 200) for 30 min at room temperature. For cell cycle analysis, the cells were stained with

(13) Inhibition of Rag GTPase signaling in mice suppresses B cell responses and lymphomagenesis with minimal detrimental trade-offsCell reportsJuly 13, 2021Ortega-Molina A, Lebrero-Fernández C, Sanz A, Deleyto-Seldas N, Plata-Gómez AB, Menéndez C, Graña-Castro O, Caleiras E, Efeyan A.Phospho-S6 Ribosomal Protein (Ser235/236) Rabbit mAb (Alexa Fluor® 647 Conjugate),Cell Signaling Technology,Clone D57.2.2E; Cat#4851; RRID:AB_10695457 APC Rat anti-Mouse IgG1,BD Biosciences,Clone A85-1; Cat#560089; RRID:AB_1645625 "CD45.1

(14) MAEA is an E3 ubiquitin ligase promoting autophagy and maintenance of haematopoietic stem cellsNature communicationsMay 4, 2021Wei Q, Pinho S, Dong S, Pierce H, Li H, Nakahara F, Xu J, Xu C, Boulais PE, Zhang D, et al.on ice, washed and stained with the following phospho-specific antibodies: pS6 (S235/236; D57.2.2E, 4851S), S6 (54D2, 55594), p4EBP1 (T37/46; 236B4), p44/42 MAPK (T202/Y204; E10, 4375S), pAKT (S473; D9E, 5315S),

(15) The delta isoform of phosphatidylinositol-3-kinase predominates in chronic myelomonocytic leukemia and can be targeted effectively with umbralisib and ruxolitinibExperimental HematologyMay 1, 2021Matthew T. Villaume et al.(Clone M89–61, Company BD:560404 and Clone D9E, CST:5315S) and pS6-Ax647 (Clone D57.2.2E, Company CST:4851S) in the dark at RT for 30 min. Cells were washed with 1mL PBS/BSA and resuspended in 0.2 mL PBS/BSA

(16) Epigenetic modulation reveals differentiation state specificity of oncogene addictionNature communicationsMarch 9, 2021Khaliq M, Manikkam M, Martinez ED, Fallahi-Sichani M.8238, AB_10839265, 1:1600), p-S6S235/S236 (rabbit mAb, clone D57.2.2E, Cell Signaling Technology, Cat# 4851, AB_10695457, 1:400), Sox10 (mouse mAb, clone SOX10/991, Abcam, Cat# ab212843, AB_2889150, 1:1200),

(17) CDK8 Fine-Tunes IL-6 Transcriptional Activities by Limiting STAT3 Resident Time at the Gene LociCell reportsDecember 22, 2020Martinez-Fabregas J, Wang L, Pohler E, Cozzani A, Wilmes S, Kazemian M, Mitra S, Moraga I.#13588S), anti-pS6R-S240/S244AF488 (Cell Signaling, #5018S), anti-pS6R-S235/S236AF647 (Cell Signaling, #4851S), anti-pZAP70-Y319/pSYK-Y352AF647 (Cell Signaling, #82975S), anti-pCREB-S133AF488 (Cell Signaling, #9187S),

(18) Lysosomal Acid Lipase Is Required for Donor T Cells to Induce Graft-versus-Host DiseaseCell reportsOctober 27, 2020Nguyen HD, Ticer T, Bastian D, Kuril S, Li H, Du H, Yan C, Yu XZ.anti-Ki67,Biolegend,#652404; RRID:N/A; Clone 16A8 Mouse anti-pS6-AF467,Cell Signaling Technology,#4851S; RRID:AB_10695457; Clone D57.2.2E CFSE,BD Biosciences,#565082; RRID:AB_2869649 Mouse anti-CD45R-biotin,Invitrogen,#13-0452-85;

(19) T cell-specific deletion of Pgam1 reveals a critical role for glycolysis in T cell responsesCommunications biologyJuly 24, 2020Toriyama K, Kuwahara M, Kondoh H, Mikawa T, Takemori N, Konishi A, Yorozuya T, Yamada T, Soga T, Shiraishi A, et al.JES6-5H4; TONBO Biosciences), anti-Phospho-S6 (Ser235/236)-Alexa Fluor 647 mAb (0.125 μg/106 cells, cat# 4851; Cell Signaling Technology, Danvers, MA, USA), anti-4E-BP1 (Thr37/46)-Alexa Fluor 488 mAb (0.05 μg/106

(20) Synthetic lethality by targeting the RUVBL1/2-TTT complex in mTORC1-hyperactive cancer cellsScience advancesJuly 1, 2020Shin SH, Lee JS, Zhang JM, Choi S, Boskovic ZV, Zhao R, Song M, Wang R, Tian J, Lee MH, et al.with anti-RUVBL2 (BD Biosciences) and anti–phospho-S6 (Alexa Fluor 647 Conjugate) (Cell Signaling, #4851) primary antibodies overnight at 4°C. After washing, Alexa Fluor 488 (Life Technologies) and Hoechst

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Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP ® Rabbit mAb (Alexa Fluor ® 647 Conjugate)

Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP ® Rabbit mAb (Alexa Fluor ® 647 Conjugate) from Cell Signaling Technology

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Cell Signaling Technology

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Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP ® Rabbit mAb (Alexa Fluor ® 647 Conjugate) from Cell Signaling Technology

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